International Travel Medicine Center of Iran International Journal of Travel Medicine and Global Health 2322-1100 10 3 2022 09 01 Investigation of Polymorphisms of ACEII Gene in People With Coronavirus With Severe and Mild Symptoms or Asymptomatic 122 126 154685 10.34172/ijtmgh.2022.22 EN Fatemeh Arab Department of Medical Genetics and Molecular Medicine, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Mozhdeh Jafari Rad Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Seyed-Alireza Esmaeili Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Immunology Department, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran 0000-0002-9371-4170 Ali Mirhosseini Immunology Research Center, Inflammation and Inflammatory Diseases Division, Mashhad University of Medical Sciences, Mashhad, Iran Mehran Moharreri Immunology Research Center, Inflammation and Inflammatory Diseases Division, Mashhad University of Medical Sciences, Mashhad, Iran Ehsan Saburi Department of Medical Genetics and Molecular Medicine, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Medical Genetic Research Center, Mashhad University of Medical Sciences, Mashhad, Iran 0000-0002-1679-1068 Journal Article 2022 06 06 <strong>Introduction:</strong> Angiotensin-converting enzyme 2 (ACE2) is the central receptor of coronavirus disease 2019 (COVID-19) in host cells. Genetic polymorphisms in the ACE2 gene may promote cardiovascular disease and systemic inflammatory injury in a patient affected by COVID-19. Thus, the genetic background may account for the substantial inter-individual diversity in illness susceptibility or severity. Our study was conducted to find a significant relationship between ACE2 rs4646142 and rs2285666 polymorphisms and susceptibility to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). <strong>Methods:</strong> In this study, we randomly selected 230 samples, including 76 patients with severe clinical symptoms and 154 patients with mild clinical symptoms (the positive case of COVID-19 was confirmed by real-time reverse transcriptase polymerase chain reaction [RTPCR] assay). Then, we performed DNA extraction and investigated the polymorphisms of rs2285666 and rs4646142 by RFLP-PCR method with TaqI and Alu1 restriction enzymes. <strong>Results:</strong> The study population included 107 men and 123 women, and the mean (±SD) age of the participants was 42.66±10.2. First, the levels of IgM and IgG were examined, and a significant association was observed in the level of IgM between the two groups of COVID-19 patients with mild and severe symptoms, as opposed to IgG. Meanwhile, no significant difference was observed between ACE2 rs4646142 and rs2285666 polymorphisms and the severity of COVID-19. <strong>Conclusion:</strong> To better understand the genetic variations in people’s susceptibility to COVID-19, this study was designed to evaluate the association between various ACE2 polymorphisms and the infection risk of SARS-CoV-2. However, no statistical difference was discovered. https://www.ijtmgh.com/article_154685_e90303163835fdac416407d2162cb30e.pdf